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KMID : 0387720100210010065
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Korean Journal of Blood Transfusion
2010 Volume.21 No. 1 p.65 ~ p.73
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Reduction of Influenza A (H1N1) Virus Through a Leukoreduction Filter
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Cho Ga-Eun
Kim Jung-Hwan
Suh In-Bum
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Abstract
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Background: Leukoreduction can reduce the risk of HLA alloimmunization, recurrent febrile nonhemolytic transfusion reactions, and several transfusion-transmitted infectious diseases, including cytomegalovirus infection. Transmission of the new influenza A (H1N1) virus through transfusion may be a concern. We evaluated the effect of filtration with a leukoreduction filter on H1N1 genomes.
Methods: To evaluate the effect of filtration by a leukoreduction filter on H1N1 genomes, we analyzed pre- and post-filtered samples from nasopharyngeal swabs and 10 positive plasma samples using real time RT-PCR.
Results: The 10 samples (nasopharyngeal swabs and plasma) contained H1N1 RNA, and filtration with a leukoreduction filter reduced these levels (threshold cycle values from 31.42±2.06 to 38.84±1.47 in nasopharyngeal swabs, from 35.63±2.19 to 39.38±2.65 in plasma samples).
Conclusion: Filtration with a leukoreduction filter can reduce H1N1 genome levels, but may not be completely sufficient for total eradication of this pathogen.
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KEYWORD
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New influenza A (H1N1) virus, RNA, Filter, Leukoreduction, Transfusion
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